Elispot and Fluorospot Assay Market Forecast

Wiki Article

The
ELISpot (Enzyme-Linked ImmunoSpot) assay is a highly sensitive
technique used to detect and quantify individual cells secreting specific
cytokines, antibodies, or other proteins. It is widely employed in
immunological research, vaccine development, and disease monitoring,
particularly in fields like cancer immunotherapy and infectious diseases. The
assay works by capturing secreted proteins from single cells onto a
membrane-coated plate using capture antibodies. After secretion and binding, a
detection antibody and enzyme-substrate reaction produce visible spots, each
corresponding to a single secreting cell. ELISpot's strength lies in its
ability to measure immune responses at the single-cell level, offering both
qualitative and quantitative insights.

The FluoroSpot assay is an advanced version of
ELISpot that uses fluorescent detection instead of enzymatic color development,
enabling the simultaneous detection of multiple cytokines or proteins from a
single cell. This multiplexing capability is crucial for studying the functional
diversity of immune responses, especially in complex disease settings like
autoimmune disorders or chronic infections. FluoroSpot uses distinct
fluorescently labeled detection antibodies for different analytes, which are
visualized using specialized imaging systems. It provides a more detailed
understanding of cellular responses by identifying polyfunctional cells—those
capable of secreting multiple cytokines—thus offering a deeper characterization
of immune cell functionality.

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